Analysis of the complete sequences of two biologically distinct Zucchini yellow mosaic virus isolates further evidences the involvement of a single amino acid in the virus pathogenicity.
نویسندگان
چکیده
The complete genome sequences of two Slovak Zucchini yellow mosaic virus isolates (ZYMV-H and ZYMV-SE04T) were determined. These isolates differ significantly in their pathogenicity, producing either severe or very mild symptoms on susceptible cucurbit hosts. The viral genome of both isolates consisted of 9593 nucleotides in size, and contained an open reading frame encoding a single polyprotein of 3080 amino acids. Despite their different biological properties, an extremely high nucleotide identity could be noted (99.8%), resulting in differences of only 5 aa, located in the HC-Pro, P3, and NIb, respectively. In silico analysis including 5 additional fully-sequenced and phylogenetically closely-related isolates known to induce different symptoms in cucurbits was performed. This suggested that the key single mutation responsible for virus pathogenicity is likely located in the N-terminal part of P3, adjacent to the PIPO.
منابع مشابه
Nucleotide sequences of the coat protein genes of two Japanese zucchini yellow mosaic virus isolates.
The nucleotide (nt) sequences of the coat protein (CP) genes of two Japanese zucchini yellow mosaic virus (ZYMV) isolates (ZYMV-169 and ZYMV-M) were determined. The CP genes of both isolates were 837 nt long and encoded 279 amino acids (aa). The nt and deduced aa sequence similarities between the two isolates were 92% and 94.6%, respectively. The deduced aa sequences of CPs of the Japanese isol...
متن کاملGenetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey
Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases of farmed rainbow trout, in Turkey, wh...
متن کاملStudy of resistance to cucumber mosaic virus watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars
متن کامل
Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey
Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trou...
متن کاملشناسایی و پراکندگی ویروس‌های آلوده کننده خیار گلخانه‌ای در منطقه جیرفت و بررسی برخی از خصوصیات فیزیکوشیمیایی ویروس موزائیک زرد کدو Zucchini yellow mosaic virus (ZYMV)
Cucumber cultivars, grown in greenhouse in the Jiroft region, were surveyed for the relative incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus-2 (WMV-2), Cucumber mosaic virus (CMV) and Tomato spotted wilt virus (TSWV) from 2001 to 2004. Samples from 1294 plants representing different cultivars were analysed by Enzyme-linked immunosorbent assay (ELISA) and Dot immunobi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Acta virologica
دوره 58 4 شماره
صفحات -
تاریخ انتشار 2014